Fig 2: Activation of Hedgehog blocks the inhibiting role of sh-KDM3A in CC. (A) Protein levels of Gli1 and Gli3 in cells determined by Western blot analysis; (B) proliferation of CC cells determined by colony formation assay; (C) apoptosis rate of cells measured by TUNEL assay; migration (D) and invasion (E) abilities of cells determined by wound-healing and Transwell assays; (F) angiogenesis ability of HUVECs determined by tube formation assay. Data were analyzed by one-way ANOVA, *P<0.05 compared to sh-KDM3A+DMSO.

Fig 3: Sh-KDM3A suppresses tumor growth in vivo. (A) Tumor volume growth in mice by time (n=5); (B) weight of tumors in mice on the 30th day; (C) expression of KDM3A, ETS1, KIF14, Gli1, and Gli3 in tissues of xenograft tumors determined by IHC staining. Data were analyzed by one-way (panel C) or two-way (panels A and B) ANOVA. *P<0.05, **P<0.01 compared to sh-NC.

Fig 4: Taz facilitates the binding of Gli3 to PKA. A-C Co-immunoprecipitation and western analyses in wild-type C3H10T1/2 cells or gene-konckout C3H10T1/2 cells (sgTaz or sgPKA) with or without N-Shh at 100 ng/ml for 24 hrs by using Gli3, Taz or PKAc antibody. A co-IP kit from Thermo company (Pierce #26149) was used to avoid detection of the IgG heavy chain. D, E Purified recombinant GST-Taz from E. coli was incubated with PKAc or Gli3F/R protein and western analyses were performed. F-H Immunoprecipitation and western analyses in HEK293 cells expressing vector, myc-Gli3 (WT) or myc-Gli3 sextuple mutant (MT) by using myc, Taz, or PKAc antibody. I-N Immunoprecipitation and western analyses in HEK293 cells expressing myc-Taz or Taz siRNA by using PKAc, Taz or Gli3 antibody. All the experiments were either duplicated or triplicated, and the representative results were shown. The mean value was present under the bands

Fig 5: An integrated working model for the cross-talk between Hippo and Hedgehog signaling. Activation of Hippo signaling (e.g. high cell density) induces the phosphorylation of Lats1/2, which in turn phosphorylate Taz, resulting in the cytosolic retention and alternatively proteasomal degradation of Taz. However, inactivation of Hippo signaling (e.g. low cell density) stabilizes cytosolic Taz, which binds to and drives the PKA to phosphorylate Gli3 at six conserved serine sites, causing the processing of full-length of Gli3 (Gli3F) into C-terminal truncation form of Gli3 (Gli3R) and thereby negating the Hedgehog signaling